Dans un contexte de contamination des eaux naturelles par des micro et nanoparticules anthropogéniques (plastiques, métaux, oxydes…), l'étude de leurs interactions avec les microalgues d’eau douce, premier maillon de la chaîne trophique, est primordiale. The unique physicochemical properties of materials at nanoscale have opened a plethora of opportunities for applications in the pharmaceutical and medical field, but also in consumer products from food and cosmetics industries. In challenge studies, HPMC films including Al2O3-NPs and SiO2-NPs at 80 ppm decreased the viability of the three-foodborne pathogens We provide high quality Silicon Dioxide E551 for food and cosmetic industries. aureus, and Salmonella Typhimurium. The cell viability was assessed by the MTT test. In this study, the nHA-MS was fabricated by a novel biphase stratification growth way. After DEX adsorption, the nanoparticles exhibited enhanced osteogenic induction in MC3T3-E1 and rat calvarial bone defect regeneration. Even though silica nanoparticle was thought to be vastly biocompatible material for use in biomedical and biotechnological applications, they could be accumulated and retained in the heart, liver, spleen, kidney, and brain after ingestion, inhalation, or skin application (Zhou et al. Compliant with the test requirements for silicon dioxide as described in the latest editions of the Food Chemicals Codex (FCC), the latest edition of Japan’s Specifications and Standards for Food Additives (D326) and European Directive 96/77/EC (monograph E551). Results: The results obtained from this study showed that the nanoparticles (~80 nm) at 80 ppm were active against Bacillus cereus, Staphylococcus aureus, and Salmonella Typhimurium compared with 20 and 40 ppm. Early indicators for nanoparticle-derived adverse health effects should provide a relative measure for cytotoxicity of nanomaterials in comparison to existing toxicological data. For developing countries, the objective is to develop drought- and pest-resistant crops, with maximized yield. Silicon Dioxide is a natural anticaking agent used as an ingredient in food and cosmetic industries. Mon travail de thèse s'est donc attaché à déterminer si les NPs, en particulier d'oxyde de zinc (ZnO), pouvaient modifier l'activité des réseaux de neurones responsables de la genèse des activités respiratoire et locomotrice. The NPs were characterized by DLS and zeta potential measurements. JRC scientists have proposed a systematic, sequential evaluation scheme involving the use of different techniques for the assessment of the presence of nanoparticles (NPs) in food-grade synthetic amorphous silica (SAS), usually coded as food additive E551. Indicators of oxidative stress and cytotoxicity, including total reactive oxygen species (ROS), glutathione, malondialdehyde, and lactate dehydrogenase, were quantitatively assessed. At nanoscale, man-made materials may show unique properties that differ from bulk and dissolved counterparts. La mise en évidence de la signature des 12 gènes représente une liste de "bio-marqueurs" potentiels et communs de la transformation cellulaire induite par les SAS, la silice cristalline Min-U-Sil 5® et le TPA. Furthermore, in vitro studies were carried out to elucidate the pathway and mechanism of in vivo damage with a co-incubation model of dopaminergic neuron PC12 and SiO(2)-NPs. Presence and risks of nanosilica in food products. However, due to their reduced particle size they are suspected to pose new risks induced by changes in biological reactivity and kinetics, which differ from those of bulk materials. The results show that microwave-assisted acidic digestion partially dissolves silica nanoparticles. We used an in vitro model to mimic the human digestion. Le test de transformation des cellules Bhas 42 a été développé afin d’évaluer les effets transformant des agents chimiques. Blocks of numbers are allocated to … Herein, we analyzed the effect of silica NPs of various sizes and surface charge on the viability of Spodoptera frugiperda cells (Sf9 cell line) with the aim of extending the knowledge of possible toxicity of the NPs in the environment and development of new tools for insect control. salt, vegetable powder, egg powder, creamer, coffee powder etc. Identifying the physico-chemical characteristics of nanoparticles (NPs) that drive their toxic activity is the key to conducting hazard assessment and guiding the design of safer nanomaterials. This is review starts with an introduction, followed by toxicity aspects, antibacterial, and testing methods and mechanisms. In the present study, the relationship between silica exposure, nitric oxide (NO) and reactive oxygen species (ROS) production, and the resultant pulmonary damage is investigated in this model. Contrary to the well-studied crystalline micron-sized silica, relatively little information exists on the toxicity of its amorphous and nano-size forms. However, another emerging, albeit under represented, area is the employment of nanoparticles as tools to understand cellular processes (e.g. The driving forces for these and future developments are the possibility to design NPs with homogeneous size and structure amenable to specific grafting. The purpose of this study is to compare the potential cytotoxicity induced by amorphous silica particles with different sizes. The safety aspect is addressed by examining recently published scientific data on the possibility of ENPs penetrating human skin. The present work aimed to assess the toxicity on DNA and cytotoxicity induced by in ovo administration of synthetic food coloring agents "sunset yellow FCF and tartrazine" with/without curcumin during the organogenesis phase of chick embryo at doses 1.575 mg/egg, 0.375 mg/egg, and 3.0 mg/kg eggs, respectively. It is generally regarded as safe and has been approved for use as a food or animal feed ingredient. Neurotoxicity was investigated in nano-SiO2-treated cultured PC12 cells, an in vitro neuronal cell model, in order to define a relatively safe dose range for its application. In addition, cytochalasin D-treatment reduced nSP70-mediated ROS generation and DNA damage, suggesting that endocytosis is involved in nSP70-mediated cellular effects. A Monte Carlo human exposure analysis to TiO(2) through foods identified children as having the highest exposures because TiO(2) content of sweets is higher than other food products and that a typical exposure for a US adult may be on the order of 1 mg Ti per kilogram body weight per day. This method may be extended to monitor the presence of SiO2 NPs in other similar complex food matrices. Cambridge: The Royal Society of Chemistry. Based on the standard curve method and other useful data analysis methods, we present and compare four statistical approaches and models for the analysis of real-time PCR data. Titanium dioxide is a common additive in many food, personal care, and other consumer products used by people, which after use can enter the sewage system and, subsequently, enter the environment as treated effluent discharged to surface waters or biosolids applied to agricultural land, incinerated wastes, or landfill solids. En conclusion, ce travail met en évidence un lien entre la formation de foyers de transformation induits par le traitement des cellules Bhas 42 avec les SAS, la silice cristalline Min-U-Sil 5® et le TPA et des modifications des profils d'expression de gènes et de voies de signalisation. Crystalline solids often cake by formation of liquid bridge and subsequent fusion of microcrystals. Further comparison of these results with in vivo data in the future would help to identify which in vitro models provide a good prediction of NM toxicity. Furthermore, SiNP surface area, surface modification and agglomeration in solution also appeared to affect oxidative potential of these SiNPs. In vivo studies have shown that liver is one of the primary target organ of silica nanoparticles. Cette technique a ensuite été utilisée pour évaluer les impacts de la présence de polluants particulaires (nanoparticules métalliques et nanoplastiques en particulier). In addition, the functionality and applicability of food-related nanotechnologies are also highlighted and critically discussed in order to provide an insight into the development and evaluation of the safety of nanotechnology in the food industry. ... Chen et al. These findings indicate that physicochemical properties are critical in influencing the oxidative behaviour of amorphous SiNPs, with potential to trigger cellular oxidative stress and thus toxicity, when exposed. Les principaux résultats de l'exposition aux nanoparticules montrent globalement la non-toxicité immédiate des nanoparticules aux concentrations testées. It’s used as an anticaking agent, emulsifier, and more. It is written with the goal of rationalizing and informing public health concerns related to this sometimes-strange new science of "nano," while raising awareness of nanomaterials' toxicity among scientists and manufacturers handling them.We show that humans have always been exposed to tiny particles via dust storms, volcanic ash, and other natural processes, and that our bodily systems are well adapted to protect us from these potentially harmful intruders. The cytotoxicity of 15-nm and 46-nm silica nanoparticles was investigated by using crystalline silica (Min-U-Sil 5) as a positive control in cultured human bronchoalveolar carcinoma-derived cells. The use of nanoparticles (NPs), including silica nanoparticles (SiO 2 -NPs), has increased intensely in the last years thanks to the technical benefits they offer [1,2]. 21, 1166-1170, Nitric Oxide and Reactive Oxygen Species Production Causes Progressive Damage in Rats after Cessation of Silica Inhalation, In Vitro Cytotoxicity of Oxide Nanoparticles: Comparison to Asbestos, Silica, and the Effect of Particle Solubility â€, In vitro toxicity of silica nanoparticles in human lung cancer cells, In Vitro Cytotoxicitiy of Silica Nanoparticles at High Concentrations Strongly Depends on the Metabolic Activity Type of the Cell Line, Development of TiO2 powder-coated food packaging film and its ability to inactivate Escherichia coli in vitro and in actual tests, Identification of Functional SNPs in BARD1 Gene and In Silico Analysis of Damaging SNPs: Based on Data Procured from dbSNP Database, Affinity of estrogens for human progesterone receptor A and B monomers and risk of breast cancer: A comparative molecular modeling study, Anti-proliferative Effects of Organic Extracts from Root Bark of Juglans Regia L. (RBJR) on MDA-MB-231 Human Breast Cancer Cells: Role of Bcl-2/Bax, Caspases and Tp53, Nanotoxicological Effects of SiO2 Nanoparticles on Spodoptera frugiperda Sf9 Cells, Mesoporous titanium dioxide@ zinc oxide–graphene oxide nanocarriers for colon-specific drug delivery. The aim of the current study is to investigate pure silica MFI-type zeolites nanoparticles with sizes of 50 nm and 100 nm (samples MFI-50 and MFI-100) under suspended conditions and their toxicological effects on human lung alveolar (A549) cells under in vitro conditions. ROS-related transcription factors and downstream cell signaling pathways are also discussed. The unique properties of engineered nanomaterials not only impart critical advantages but also confer toxicity because of their unwanted interactions with different biological compartments and cellular processes. For the first scenario no adverse effects are expected to occur. Treatment with a high concentration of E551 caused significant cytotoxic effects on WI-38 cells. Iron oxide ENM was dispersed in water by sonication and combined with the food model. Our study results clearly indicate that sulforaphane can protect hMSCs against Cd-induced changes in nuclear morphology, attenuation of MMP, and alteration of gene expression patterns. JRC scientists have proposed a systematic, sequential evaluation scheme involving the use of different techniques for the assessment of the presence of nanoparticles (NPs) in food-grade synthetic amorphous silica (SAS), usually coded as food additive E551. Food additives permitted in the EU: compounds used as anticaking agents and other uses. This result may have an impact on the evaluation of whether the material is a nanomaterial according to the recommended definition of the European Commission. It has been designated “generally regarded as safe” (GRAS). Nanoparticles, the building blocks of nanotechnology, have been widely utilized in various biomedical applications, such as detection, diagnosis, imaging, and therapy. Other than a 10% increases over controls in reactive oxygen species (ROS) production with 1.5% w/w CNC, no significant changes in cytotoxicity, ROS or monolayer integrity were observed. Design of the Study: *This study was designed using two nanoparticles i.e. This paper presents an effective approach for both size characterization and mass quantification of SiO2 NPs in commercial high-fat coffee creamer using asymmetric flow field-flow fractionation (AF4) coupled to inductively coupled plasma mass spectrometry (ICP-MS). Tumor necrosis factor alpha inhibits CYP1A1 expression, and this inhibition is prevented by the antioxidant pyrrolidine dithiocarbamate. This chapter contains sections titled: Introduction, Silicon dioxide (SiO2) has been used as a food additive (E551) for decades. 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